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In Vitro Screening for Cytotoxic Activity of Herbal Extracts

To demonstrate the potential biological activity of alcoholic extracts, we carried out a screening of the extracts by measuring their in vitro antiproliferative properties against several common human cancer cell lin Some plant extracts exhibited antiproliferative activity toward all ,...

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Anticancer activity and chromatography characterization of ,

09-04-2013 0183 32 Screening of anticancer activity , mice 10 mice for each set of experiment , by passaging every 10 days EAC cells of 9 day old were used for the screening Plant extracts IC50 values of each plant extracts in , Overnight nutrient broth culture of the test organisms was seeded over the Nutrient agar NA plates using sterile ....

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Phytochemical screening and anticancer activity of the ,

23-01-2021 0183 32 Cancer is one of the most considerable concerns because of increasing the death rate all over the world Recent studies have disclosed that plant extracts exhibit anticancer activity through various mechanisms Xanthium strumarium has been used by Vietnamese in herbal medicines to support the medication of infirmiti This study is to consider the ,...

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Anticancer activity of plant leaves extract collected from ,

12-10-2019 0183 32 The goal of this research was to explore the preliminary anticancer properties of five plants namely Calotropis procera, Moringa oleifera, Millettia pinnata, Basela alba and Euphorbia neriifolia available in Jharkhand which is used for the medicinal purpose by local trib In the present study, plant leaves from five species were collected, dried and extracted with ,...

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Phytochemical analysis and evaluation of anticancer ,

Phytochemical screening of the plant extracts Phytochemical screening for the presence of alkaloids, anthraquinones, flavonoids, saponins, tannins, sterols, reducing sugars, glycosides, and terpenes in the extracts was carried following the protocol ,...

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Extracts of select endemic plants from the Republic of ,

19-02-2021 0183 32 So far, Mauritius endemic plant families Euphorbiaceae Acalypha integrifolia Willd and Myrtaceae Eugenia tinifolia Lam have been screened for their anti-cancer properties they were shown to be cytotoxic against human oesophageal squamous cell carcinoma KYSE-30 at IC50 of 642 177 221 and 699 177 038 0 181 g LW/ml of extracts respectively 14...

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In vitro Anticancer Screening of 24 Locally Used Nigerian ,

Methods The antitumor activity of methanolic extracts obtained from 24 of the selected plants, were evaluated in vitro on five human cancer cell lin Results Results obtained from the plants screened indicate that 18 plant extracts of folk medicine exhibited promising cytotoxic activity against human carcinoma cell lin...

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Article Screening for Selective Anticancer Activity of ,

screening for selective anticancer activity of 57 extracts from 45 plants collected in Grazalema Natural Park, an area in the South of Spain of high plant diversity and endemism Using lung cancer cells A549 and lung non-malignant cells MRC ,...

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Cytotoxicity screening of Bangladeshi medicinal plant ,

17-09-2010 0183 32 Cytotoxicity screening using PC biosensor-based cell attachment assay The preliminary determination of the effect of each plant extract on Panc-1 cells was made in an initial screen using a label-free PC biosensor-based assay Details of the PC biosensor and imaging instrumentation have been described 12, 13...

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screening of anticancer plant extracts using culture

screening of anticancer plant extracts using culture SCREENING OF SOLANUM NIGRUM FOR ITS ,, pure culture All the bacteria were isolated and identified using morphological and biochemical tests adopting standard procedures Cowan and Steel, 1974 Stock culture was maintained in both Agar slant and 20 sterile buffered glycerol Phytochemical Screening The ,...

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ANTICANCER ACTIVITY OF EXCOECARIA AGALLOCHA LEAF EXTRACT ,

ANTICANCER ACTIVITY OF EXCOECARIA AGALLOCHA LEAF EXTRACT IN CELL LINE MODEL , The present study was aimed at using leaf crude extract , 1 Monolayer cultures in log phase 2 Drug extract different concentrations 3 MEM without FCS 4 045 181 filter 5...

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Biological screening of various medicinal plant extracts ,

screening and testing plant extracts 4 Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1 New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5 Increasingly, natural products have potential for the treatment of cancer...

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Screening of Microbial Extracts for Anticancer Compounds ,

01-05-2018 0183 32 Title of article - Screening of Microbial Extracts for Anticancer Compounds Using Streptomyces Kinase Inhibitor Assay Abstract Eukaryotic kinases are known to play an important role in signal transduction pathways by phosphorylating their respective substrat...

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Antimicrobial activity, cytotoxicity and phytochemical ,

information on the activity of the plant extracts against a broad range of microorganisms Furthermore, the plant extracts were subjected to preliminary phytochemical screening to analyze the possible antimicrobial compounds they contain The study provides scientific evidence on the possible use of these plants to produce...

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Which method is most suitable and accurate for screening ,

We want to screen pant extracts for anticancer activity by using cell culture or cell line We are looking for the best ....

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Study of the Anticancer Potential of Plant Extracts Using ,

The primary objective of the present study was to utilize a liver tumor MPS for testing the anticancer activities of plant extracts and their comparison with the traditional cell culture model The anticancer activity of methanolic extracts of Acer cappadocicum Gled was tested against the liver tumor MPS composed of hepatocellular carcinoma cells...

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Evaluation of antimicrobial activity of different solvent ,

studies reported the anticancer 15, antimalarial activity, analgesic and , medicinal importance, the present protocol has been regarding the outlined antimicrobial activity on these selected plant using different extracts It is in view of this, that the present research was set up to , Culture and Maintenance of microorganisms...

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In vitro wound healing potency of methanolic leaf extract ,

01-05-2019 0183 32 Besides plant extracts, herbal formulations made of a combination of plant extracts have also been tested using these cell lines, particularly the L929 cells In other words, the migratory and proliferative abilities of the fibroblasts are pivotal to wound healing...

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Article Screening for selective anticancer activity of 65 ,

12-06-2018 0183 32 1 Article Screening for selective anticancer activity of 65 extracts of plants collected in Western Andalusia, Spain José Manuel Calderón-Montaño1, Sara María Martínez-Sánchez2, Estefanía Burgos-Morón1, Emilio Guillén-Mancina1, Julio José Jiménez-Alonso1, Felipe García2, Abelardo Aparicio2, Miguel López- ....

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Antitumor activity of plant extracts pdf

Antitumor activity of plant extracts pdf Abstract Introduction The monolayer cell culture model is a popular model for screening anti-tumor activity of plant extracts However, almost the extracts selected for screening in this model have failed in subsequent animal models...

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In vitro anticancer screening of South African plants ,

28-10-2008 0183 32 Plant extracts that exhibited anticancer activity against these three human cell lines were screened by the NCI against sixty human cancer cell lines organized into sub-panels representing leukaemia, melanoma, cancer of the lung, colon, kidney, ovary, central nervous system, breast and prostate Results...

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SCREENING OF FUNGAL CULTURES FOR ANTI

Extracts are also provided to ARS for in-house screening purposes for potential use in agriculture, in addition to information concerning antimicrobial activiti From a core collection of insect pathogenic fungi, over 53 of the fungi screened were shown to ,...

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Cell Culture Based in vitro Test Systems for Anticancer ,

The development of new high-tech systems for screening anticancer drugs is one of the main problems of preclinical screening Poor correlation between preclinical in vitro and in vivo data with clinical trials remains a major concern The choice of the correct tumor model at the stage of in vitro testing provides reduction in both financial and time costs during later stages due to ,...

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In vitro cytotoxic activity of medicinal plants from ,

22-11-2017 0183 32 The present study was aimed at determining the cytotoxicities of 31 medicinal plants extracts, used in Nigerian ethnomedicine for the treatment of cancer Methods The plant extracts were screened for cytotoxicity, using the brine shrimp ,...

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In vitro anticancer activity and cytotoxicity screening of ,

extract EAE , n-butanol extract BE , the remainder after extraction RE and water extract WE from each plant were prepared These 60 extracts were screened for their cytotoxicity on the aforementioned cell lines using MTT assay Results All plant species showed certain anticancer activi-...

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Which is the best method for in screening for anticancer ,

07-10-2020 0183 32 You can use MTT or MTS or SRB methods to test the anticancer activity of a pant extract in cell culture regards Cite 1 Recommendation 19th Aug, 2018 Shankara Narayanan Subramaniam SASTRA....

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20 MATERIALS AND METHODS 21 Plant material and extract ,

23 Antimicrobial susceptibility testing 231 , culture were suspended in four to five ml of Mueller Hinton broth and incubated at 37 , The discs which had been impregnated with plant extracts using sterile forceps were applied on the inoculated Mueller Hinton agar once it has completely dried...

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In vitro anticancer activity and cytotoxicity screening of ,

In vitro anticancer activity and cytotoxicity screening of phytochemical extracts from selected traditional Chinese medicinal plants Our results provided new evidence for anticancer activities of these plants which could be useful for developing new anticancer therapi...

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